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A common buffer in biochemistry work is a phosphate buffer.

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January 1st, 2018

A common buffer in biochemistry work is a phosphate buffer. it uses the following equilibrium:H2PO4- + H2O –> HPO42-+ H3O+ pKa= 7.2For 1 L of a 20 mM phosphate buffer at pH 7.4, what is the pH after adding 1M of 1M HCl?i understand you have to use pH=pka + log [A-]/[Ha]to find the moles of HA but i don’t understand what happens after

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